(461) Endocytic Uptake of Quantum Dots and Endocytic Markers into `Sweet Lime' Juice Cells

Endocytic Uptake of Nutrients, Cell Wall Molecules, and Fluidized Cell Wall Portions into Heterotrophic Plant Cells

After arrival at the surface of heterotrophic cells, nutrients are taken up by these cells via endocytosis to sustain metabolic processes. Recent advances in plant endocytosis reveal that this is true for their heterotrophic cells, either cultivated in suspension cultures or for intact root apices. Importantly, sucrose appears to act as a specific stimulus for fluid-phase endocytosis. Uptake of...

Fluid phase endocytic uptake of artificial nano-spheres and fluorescent quantum dots by sycamore cultured cells: evidence for the distribution of solutes to different intracellular compartments.

Fluid phase endocytic uptake of external solutes in plant cells was further substantiated using artificial polystyrene nano-spheres (40 nm) and CdSe/ZnS quantum dots (20 nm). Both types of artificial nano-particles were taken up by sycamore-cultured cells. However, whereas polystyrene nano-spheres were delivered to the central vacuole, CdSe/ZnS nano-dots were sequestered into cytoplasmic vesicu...

Fluorescence-Lifetime Imaging Microscopy for Visualization of Quantum Dots’ Endocytic Pathway

Accumulation of carboxylated polyethylene glycol (PEG) CdSe/ZnSquantum dots (QDs) has been monitored in living fibroblasts using confocal microscopy for fluorescence intensity and fluorescence-lifetime imaging (FLIM). The wide range of mean photoluminescence (PL) lifetime values was observed for the intracellular QDs in different intracellular microenvironment, which revealed structural heterog...

Fusion accessibility of endocytic compartments along the recycling and lysosomal endocytic pathways in intact cells

A fluorescence assay developed for the quantitation of intracellular fusion of sequentially formed endocytic compartments (Salzman, N. H., and F. R. Maxfield. 1988 J. Cell Biol. 106:1083-1091) has been used to measure the time course of endosome fusion accessibility along the recycling and degradative endocytic pathways. Transferrin (Tf) was used to label the recycling pathway, and alpha2-macro...

Endocytic Uptake into Mammalian Cells during Antigen and Binding of Lethal Factor Oligomerization of Anthrax Toxin Protective